27. Tsaousis, A. D. (2019) On the origin of Fe/S cluster biosynthesis in eukaryotes. Frontiers in Microbiology. Accepted. doi: 10.3389/fmicb.2019.02478 

26. Rueckert, S., Betts, E. and Tsaousis, A. D.* (2019). The symbiotic spectrum: where do the gregarines fit?. Trends In Parasitology. In press  

25. Robertson, L.J., Clark C.G., Debenham J.J., Dubey, J.P., Kváč, M., Lig, J., Ponce-Gordo F., Ryan, U., Schares G., Suk, C., Tsaousis, A.D., (2019) Are molecular tools clarifying or confusing our understanding of the public health threat from zoonotic enteric protozoa in wildlife? . Int J Parasitol. Parasites & Wildlife.

24. Li FJ, Tsaousis AD, Purton T, Chow VTK, He CY, Tan KSW. (2019) Successful Genetic Transfection of the Colonic Protistan Parasite Blastocystis for Reliable Expression of Ectopic Genes. Sci Rep. 2019 Feb 28;9(1):3159. doi: 10.1038/s41598-019-39094-5. 

23. Jossé L, Bones AJ, Purton T, Michaelis M, Tsaousis AD. A Cell Culture Platform for the Cultivation of Cryptosporidium parvum. Curr Protoc Microbiol. 2019 Feb 8:e80. doi: 10.1002/cpmc.80.

22. Bones AJ, Jossé L, More C, Miller CN, Michaelis M, Tsaousis AD.Past and future trends of Cryptoporidium in vitro research. Exp Parasitol. 2019 Jan;196:28-37. doi: 10.1016/j.exppara.2018.12.001. Epub 2018 Dec 3.

21. Miller C. N., Panagos H., Mosedale W.R.T., Kvac M., Howard M. J. and Tsaousis A. D.*(2019) NMR metabolomics reveals effects of Cryptosporidiuminfections on host cell metabolome Gut Pathog April 3; 11:13.


20. Tsaousis A.D., Hamblin K.A., Elliot C.R., Young L., Rosell-Hidalgo A.,  Gourlay C., Moore A.L., & van der Giezen M., (2018) The Human Gut Colonizer Blastocystis Respires Using Complex II and Alternative Oxidase to Buffer Transient Oxygen Fluctuations in the Gut. Front Cell Infect Microbiol. 2018 Oct 22;8:371. doi: 10.3389/fcimb.2018.00371.

19. Miller C. N., Josse L. J., and Tsaousis A. D. (2018) Localization of Fe-S biosynthesis machinery in Cryptosporidium parvum mitosome, J Eukaryot Microbiol. 2018 Jun 22. doi: 10.1111/jeu.12663.

18. Yowang A., Tsaousis A. D., Chumphonsuk T., Thongsin N., Kullawong N., Popluechai S., Gentekaki E. (2018) High diversity and variable geographic distribution of Blastocystis subtypes isolated from adults living in Thailand. Infect Genet Evol. 2018 Aug 15;65:270-275. doi: 10.1016/j.meegid.2018.08.010.

17. Herman E. K., Yiangou L., Cantoni D. M., Miller C. N., Marciano-Carbal F., Anthonyrajah E., Dacks J. B., Tsaousis A. D., (2018) Identification and characterisation of the cryptic Golgi Apparatus in Naegleria gruberi. J Cell Sci. 2018 Apr 9;131(7).

16. Betts E. L., Gentekaki E., Thomasz A., Breakell V., Carpenter A., Tsaousis A. D. (2018) Genetic diversity of Blastocystis in non-primate animals. Parasitology, 1-7. doi:10.1017/S0031182017002347

15. Miller C. N., Josse L. J., Brown I., Blakeman, B. J. F., and Povey J. F., Nader, Price M., Cinatl J., Xue W-F., Michaelis M., and Tsaousis A. D. (2018) A new platform that enables long-term cultivation of Cryptosporidium. Int J Parasitol. 2018 Mar;48(3-4):197-201. doi: 10.1016/j.ijpara.2017.10.001.

F1000 recommendation:  


14. Rueckert S., Tsaousis A. D., (2017) Conference: Report of the 2017 Protistology-UK Spring Meeting, European Journal of Protistology, 61, 307 – 310 

13. Gentekaki, E., Curtis, B.A., Stairs, C.W., Klimeš, V., Eliáš, M., Salas-Leiva, D.E., Herman, E.K., Eme, L., Arias, M.C., Henrissat, B. Hilliou, F., Klute, M.J., Suga, H.,Malik, S.B., Pightling, W.W., Kolisko, M., Rachubinski, R.A., Schlacht, A., Soanes, D.M., Tsaousis, A. D., Archibald, J.M., Ball, S.G., Dacks, J.B., Clark, C.G., van der Giezen, M., Roger A.J. (2017) Extreme genome diversity in the hyper-prevalent parasitic eukaryote Blastocystis. PLoS Biol, 15(9):e2003769. doi: 10.1371/journal.pbio.2003769

12. Saintas E, Abrahams L, Ahmad GT, Ajakaiye AM, AlHumaidi AS, Ashmore-Harris C, Clark I, Dura UK, Fixmer CN, Ike-Morris C, Mato Prado M, Mccullough D, Mishra S, Schöler KM, Timur H, Williamson MD, Alatsatianos M, Bahsoun B, Blackburn E, Hogwood CE, Lithgow PE, Rowe M, Yiangou L, Rothweiler F, Cinatl J Jr, Zehner R, Baines AJ, Garrett MD, Gourlay CW, Griffin DK, Gullick WJ, Hargreaves E, Howard MJ, Lloyd DR, Rossman JS, Smales CM, Tsaousis A.D., von der Haar T, Wass MN, Michaelis M. Acquired resistance to oxaliplatin is not directly associated with increased resistance to DNA damage in SK-N-ASrOXALI4000, a newly established oxaliplatin-resistant sub-line of the neuroblastoma cell line SK-N-AS. PLoS One.  2017 Feb 13;12(2)



11. Richardson E., Zerr K, Tsaousis A.D., Dorrell R.G., & Dacks J.B., (2015) Evolutionary cell biology: functional insight from "endless forms most beautiful". Mol Biol Cell. 2015 Dec 15;26(25):4532-8. LINK


10. Tsaousis A.D., Nývltová E., Sutak, R., Hrdy, I., & Tachezy J. (2014) Non-mitochondial hydrogen production of Naegleria gruberi. Gen Biol Evol 6(4), 792-799. LINK

9. Tsaousis A.D., Gentekaki E., Eme L., Gaston D. & Roger A.J. (2014) Evolutionary relationships and origin of the Cytosolic Iron-Sulphur Assembly (CIA) machinery in eukaryotes. Eukaryotic Cell, 13(1): 143-53. LINK


8. Tsaousis A. D., Ollagnier-de Choudens S., Gentekaki E., Long S., Gaston D., Stechmann A., Vinella D., Py B., Fontecave M., Barras F., Lukeš J., & Roger A. J., (2012) Evolution of Fe-S cluster biogenesis in the anaerobic parasite Blastocystis, Proc Natl Acad Sci USA 109(26):10426-31. LINK



7. Tsaousis, A. D., Gaston D., Stechmann A., Walker P. B., Lithgow T. & Roger A. J. (2011) A Functional Tom70 in the Human Parasite Blastocystis sp.: Implications for the Evolution of the Mitochondrial Import Apparatus, Mol Biol Evol 28(1):781-91. LINK


6. Long S, Changmai P, Tsaousis AD, Skalický T, Verner Z, Wen YZ, Roger AJ, Lukeš J. Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues. Mol Microbiol. 2011 Sep;81(6):1403-18. doi:

10.1111/j.1365-2958.2011.07769.x. LINK




5. Hjort, K., Goldberg, A.V., Tsaousis, A.D., Hirt, R.P., and Embley, T.M. (2010). Diversity and reductive evolution of mitochondria among microbial eukaryotes. Philos Trans R Soc Lond B Biol Sci 365, 713-727. LINK




4. Gaston D., Tsaousis A. D., & Roger A. J. (2009) Predicting proteomes of mitochondria and related organelles from genomic and expressed sequence tag data, Methods in Enzymol, 457: 21-41. LINK



3. Tsaousis, A. D., Kunji E. R. S., Goldberg, A. V., Lucocq J. M., Hirt, R. P. & Embley T. M. (2008) A novel route for ATP acquisition by the remnant mitochondria of Encephalitozoon cuniculi, Nature 453: 553-556. LINK

Faculty of 1000 Biology: evaluations for Tsaousis AD et al Nature 2008 May 22 453 (7194) :553-6 - Factor 8.0 - Exceptional


2. Goldberg, A. V., Molik, S., Tsaousis, A. D., Kuhnke, G., Delbac, F., Vivares, C., Hirt, R. P., Lill, R. & Embley T. M. (2008) Localisation and functionality of microsporidian iron-sulphur cluster assembly proteins, Nature 452: 624-628. LINK




1. Tsaousis, A. D., Martin, D. P., Ladoukakis, E. D., Posada, D. & Zouros, E. (2005) Widespread Recombination in Published Animal mtDNA Sequences, Mol Biol Evol 22: 925-933. LINK