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58. Roblin, M., Canniere, E., Barbier, A., Daandels, Y., Dellevoet-Groenewegen, M., Pinto, P., Tsaousis, A.D. and Leruste, H., Brainard, J., & Follet, J. (2023), Study of the Economic Impact of Cryptosporidiosis in Calves after Implementing Good Practices to Manage the Disease on Dairy Farms in Belgium, France, and the Netherlands. Accepted. Available at SSRN:


57. Chengan, K., Hind, C., Nagappa, L., Wand, M. E., Hanson, T., Martín-Escolano R., Tsaousis, A.D., Bengoechea, J.A., Sutton, J.M., Smales, C.M., Moore, S.J., (2023) A cell-free strategy for profiling intracellular antibiotic sensitivity and resistance. npj Antimicrobials and Resistance, bioRxiv 2023.04.13.536698; doi:


56. Záhonová K., Low R., Warren C.J., Cantoni D. M., Herman E.K., Yiangou Y., Ribeiro C.A., Phanprasert Y., Brown I.R., Rueckert S.,  Baker N.L., Tachezy J.,  Betts E.L., Gentekaki E., van der Giezen M., Clark C.G., Jackson A.P., Dacks J.B. and Tsaousis A.D.* (2023) Evolutionary cell biological analysis of Proteromonas reveals the path to cellular reduction and anaerobicity in the hyper-prevalent gut microbe Blastocystis. Current Biology, Vol: 33, 12, Page: 2449-2464.e8. 


55. Hoque, S., Pinto P., Azevedo-Ribeiro C., Canniere E., Dellevoet M., Bourgeois A., Daandels Y., Hammouma, O., Gentekaki E., Kvac M., Follet J., and Tsaousis, A.D.* (2023) Follow-up investigation into Cryptosporidium prevalence and transmission in Western European dairy farms Vet Parasitol. 2023 Jun;318:109920. doi: 10.1016/j.vetpar.2023.109920.(Impact factor: 2.821)


54. McCain A., Gruneck L., Popluechai C., Tsaousis A.D.* and  Gentekaki E. (2023) Blastocystis high prevalence, stable colonization, transmission dynamics and links to demographic and anthropometric variables among schoolchildren of various ethnicities in rural northern Thailand. Epidemiology & Infection, 151, E77. doi:10.1017/S0950268823000596 (Impact factor: 4.434; cited by 1 article)


53. Maxamhud S., Reghaissia N., Laatamna A., Samari H., Remdani N., Gentekaki E., Tsaousis A.D.* Molecular Identification of Cryptosporidium spp., and Giardia duodenalis in Dromedary Camels (Camelus dromedarius) from the Algerian Sahara. Parasitologia. 2023; 3(2):151-159.


52. Jinatham V., Wandee T., Nonebudsri C., Popluechai C., Tsaousis A.D. and Gentekaki E. (2023) Blastocystis in raw vegetables from street markets in northern Thailand. Parasitol Res 2023 Apr;122(4):1027-1031. doi: 10.1007/s00436-023-07781-y. (Impact factor: 2.383)


51. Maloney J.G., Molokin A., Seguí R., Maravilla P., Martínez-Hernández F., Villalobos G., Tsaousis A.D., Gentekaki E., Muñoz-Antolí C., Klisiowicz D.R., Oishi C.Y., Toledo R., Esteban J.G., Köster P.C., de Lucio A., Dashti A., Bailo B., Calero-Bernal R., González-Barrio D., Carmena D., and Santín M. (2022) Identification and molecular characterization of four new Blastocystis subtypes designated ST35-ST38. Microorganisms. 2023, 11, 46.


50. Martín-Escolano R., Ng G.C., Tan K. S. W., Gentekaki E., and Tsaousis A.D.* (2022) Blastocystis resistance in chlorine and hydrogen peroxide. Parasitol Res. 2023 Jan;122(1):167-176. doi: 10.1007/s00436-022-07713-2. 


49. Henriquez F., Rueckert S., Tsaousis A.D. (2022) Protists on the sport: opening the field of view on Protistology. Microbiology Today, October 2022 issue.


48. Mckinlay K., Betts E.L., Tsaousis A.D., Rueckert S. (2022) Gregarine apicomplexans – model organisms to uncover the evolutionary path to obligate intracellular parasitism? Microbiology Today, October 2022 issue.


47. Jinatham V., Nonebudsri C., Wandee T., Popluechai S., Tsaousis A.D., Gentekaki E. (2022) Blastocystis in tap water of a community in northern Thailand. Parasitol Int. 2022 Dec;91:102624. doi: 10.1016/j.parint.2022.102624. (Impact factor:2.106)


46. Reghaissia N, Maxamhud S, Laatamna A, Samari H, Dahmane A, Berima R, Abdelli A, Hakem A, Baroudi D, Tsaousis A.D. (2022) First Epidemiological Report on the Prevalence and Associated Risk Factors of Cryptosporidium spp. in Farmed Marine and Wild Freshwater Fish in Central and Eastern of Algeria. Acta Parasitol. 2022 Sep;67(3):1152-1161. doi: 10.1007/s11686-022-00560-2. 


45. Martín-Escolano J., Marín C., Rosales M., Tsaousis, A. D., Medina-Carmona E., and Martín-Escolano, R. (2022) Shifting View of the Life Cycle of Trypanosoma cruzi: a Challenge for an Effective Treatment. ACS Infectious Diseases, Jun 10;8(6):1107-1115. 


44. Cantoni D.M., Osborne A., Taib N. Thompson G. S., Escolano R.M., Kazana E., Edrich E., Brown I. R., Gribaldo S., Gourlay C.W. and Tsaousis A.D.* (2022), Localization and functional characterisation of Alternative Oxidase in Naegleria. Journal of Eukaryotic Microbiology. Accepted 


43.  Hoque S., Mavrides D., Pinto P., Costas, S., Begum N., Azevedo-Ribeiro C., Liapi M., Malas S., Kvac M., Gentekaki E. and Tsaousis A. D.*, (2022) High occurrence of zoonotic subtypes of Cryptosporidium parvum in Cypriot dairy farms. Microorganisms 2022, 10, 531. Doi: 10.3390/microorganisms10030531 (Impact factor: 4.926)



42. Escolano R.M., Yiangou L., Kazana E., Robinson G., Michaelis M., and Tsaousis A.D.* (2021) Repurposing in vitro approaches for screening anti-parasitic drugs against the brain-eating amoeba Naegleria fowleri.  Int J Parasitol Drugs Drug Resist. 2021 Dec;17:204-212. doi: 10.1016/j.ijpddr.2021.10.003. Epub 2021 Nov 25. 


41. Pinto P., Hoque, S., Azevedo-Ribeiro C., Wuylsteke I., Canniere E., Dellevoet M., Bourgeois A., Leruste H., Roblin M., Windal F., Deweer C., Robinson G., Daandels Y., Kvac M., Follet J., and Tsaousis, A.D.* (2021) Cross-border investigations on the prevalence and transmission dynamics of Cryptosporidium species in dairy cattle farms in western mainland Europe. Microorganisms. 2021 Nov 20;9(11):2394. doi: 10.3390/microorganisms9112394

40. Betts E.L., Newton J. M., Thompson G.S., Sarzahanov F., Jinatham V., Kim M.-J., Popluechai S., Dogruman-Al F., Won E.J., Gentekaki E.* and Tsaousis A. D.* (2021) Metabolic fluctuations in the human stool obtained from Blastocystiscarriers and non-carriers. Metabolites. 2021 Dec 17;11(12):883. doi: 10.3390/metabo11120883. 


39. Jinatham V., Maxamhud S., Popluechai S., Tsaousis A. D*., and Gentekaki E.* (2021) Blastocystis One Health approach in a rural community of northern Thailand: prevalence, subtypes and novel transmission routes. Front Microbiol. 2021 Dec 9;12:746340. doi: 10.3389/fmicb.2021.746340.  


38. Jinatham V., Cantoni D. M., Phanprasert Y., Vichaslip T., Suwannahitatorn P., Popluechai S., Tsaousis A. D*., and Gentekaki E.* (2021) Prototheca bovis, a unicellular achlorophyllous trebouxiophyte green alga in the healthy human intestine. J Med Microbiol. 2021 Sep;70(9). doi: 10.1099/jmm.0.001415 


37. Tsaousis A. D.*, Gentekaki E., Stensvold C. R., Tan K.S.W., Dogruman-Al F.,and Kurt O., (2021) TrendsTalk: The 3rd International Blastocystis Conference: coming together virtually. Trends in Parasitology. (Impact factor: 9.014)

36.    Newton J. M., Betts E.L., Yiangou L., Ortega-Roldan J., Tsaousis A.D.* and Thompson G.S.*, (2021) Establishing a metabolite extraction method to study the metabolome of Blastocystis. Molecules. 26, no. 11: 3285.


35. Betts E. L., Hoque S., Torbe L., Baily J.L., Ryan H. Toller K., Breakell V., Carpenter A.I., Diana A., Matechou E., Gentekaki E., and Tsaousis A. D.* (2021) Parasites, Drugs and Captivity: Blastocystis-microbiome associations in captive water voles. Biology, 2021; 10(6):457.


34.    Herman E.K., Greninger A., van der Giezen M., Ginger M.L., Ramirez-Macias I., Miller H.C., Morgan M.J., Tsaousis A.D., Velle K., Vargová R., Rodrigo Najle S., MacIntyre G., Muller N., Wittwer M., Zysset-Burri D.C., Elias M., Slamovits C., Weirauch M., Fritz-Laylin L., Marciano-Cabral F., Puzon G.J., Walsh T., Chiu C. and Dacks J.B. (2021), Genomics and transcriptomics yields a system-level view of the biology of the pathogen Naegleria fowleri. BMC Biol 19, 142 (2021). 


33.   Rueckert S., Henriquez F., Bass D., and Tsaousis A.D., (2021) The increase in global aquaculture and the associated protists: opportunities and threats. Microbiology Today. May 2021 


32.   Pyrih J., Pánek T., Cimrhanzlová K., Rašková V., Kriegová E., Tsaousis A.D., Eliáš M., Lukeš J., (2021) Vestiges of the Bacterial Signal Recognition Particle-Based Protein Targeting in Mitochondria. Molecular Biology and Evolution, Volume 38, Issue 8, August 2021, Pages 3170–3187,


31.    Horváthová, L., Žárský, V., Pánek, T., Derelle, R., Pyrih, J., Motyčková, A., Klápšťová, V., Klimeš, V., Petrů, M., Vaitová, Z., Čepička, I., Harant, K., Gray, M., Guilvout, I., Francetic, O., Lang, B., Vlček, Č, Tsaousis, A. D., Eliáš, M. and Doležal, P. (2021). Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system. Nature Communications. 12, 2947. 


30.    Mirzaee Baftia, S., Anga, C.S., Hossain, M. M., Marcelli, G., Alemany-Fornes M., and Tsaousis, A.D., (2021) A crowdsourcing semi-automatic image segmentation platform in cell biology. Computers in biology and Medicine. 130, 104204



29.   Faktorová, D., Nisbet, E., Fernández Robledo, J., Casacuberta, E., Sudek, L., Allen, A., Ares Jr., M., Aresté, C., Balestreri, C., Barbrook, A., Beardslee, P., Bender, S., Booth, D., Bouget, F., Bowler, C., Breglia, S., Broellochs, A., Brownlee, C., Burger, G., Cerutti, H., Cesaroni, R., Chiurillo, M., Clemente, T., Coles, D., Collier, J., Cooney, L., Coyne, K., Docampo, R., Dupont, C., Edgcomb, V., Einarsson, E., Elustondo, P., Federici, F., Freire-Benéitez, V., Freyria, N., Fukuda, K., García, P., Girguis, P., Gomaa, F., Gornik, S., Guo, J., Hampl, V., Hanawa, Y., Haro-Contreras, E., Hehenberger, E., Highfield, A., Hirakawa, Y., Hopes, A., Howe, C., Hu, I., Ibañez, J., Irwin, N., Ishii, Y., Janowicz, N., Jones, A., Kachale, A., Fujimura-Kamada, K., Kaur, B., Kaye, J., Kazana, E., Keeling, P., King, N., Klobutcher, L., Lander, N., Lassidi, I., Li, Z., Lin, S., Lozano, J., Luan, F., Maruyama, S., Matute, T., Miceli, C., Minagawa, J., Moosburner, M., Najle, S., Nanjappa, D., Nimmo, I., Noble, L., Novák Vanclová, A., Nowacki, M., Nuñez, I., Pain, A., Piersanti, A., Pucciarelli, S., Pyrih, J., Rest, J., Rius, M., Robertson, D., Ruaud, A., Ruiz-Trillo, I., Sigg, M., Silver, P., Slamovits, C., Swart, E., Smith, G., Sprecher, B., Stern, R., von der Haar, T., Tsypin, L., Turkewitz, A., Turnšek, J., Valach, M., Vergé, V., von Dassow, P., Tsaousis, A. D., Waller, R., Wang, L., Wen, X., Wheeler, G., Woods, A., Zhang, H., Mock, T., Worden, A. and Lukeš, J. (2020). Genetic tool development in marine protists: Emerging model organisms for experimental cell biology. Nature Methods. 17, 481–494 


28.    Betts E. L., Gentekaki E., Tsaousis A. D. (2020) Exploring Micro-Eukaryotic Diversity in the Gut: Co-occurrence of Blastocystis Subtypes and Other Protists in Zoo Animals. Frontiers in Microbiology. Vol 11. 



27. Tsaousis, A. D. (2019) On the origin of Fe/S cluster biosynthesis in eukaryotes. Frontiers in Microbiology. Accepted. doi: 10.3389/fmicb.2019.02478 

26. Rueckert, S., Betts, E. and Tsaousis, A. D.* (2019). The symbiotic spectrum: where do the gregarines fit?. Trends In Parasitology. In press  

25. Robertson, L.J., Clark C.G., Debenham J.J., Dubey, J.P., Kváč, M., Lig, J., Ponce-Gordo F., Ryan, U., Schares G., Suk, C., Tsaousis, A.D., (2019) Are molecular tools clarifying or confusing our understanding of the public health threat from zoonotic enteric protozoa in wildlife? . Int J Parasitol. Parasites & Wildlife.

24. Li FJ, Tsaousis AD, Purton T, Chow VTK, He CY, Tan KSW. (2019) Successful Genetic Transfection of the Colonic Protistan Parasite Blastocystis for Reliable Expression of Ectopic Genes. Sci Rep. 2019 Feb 28;9(1):3159. doi: 10.1038/s41598-019-39094-5. 

23. Jossé L, Bones AJ, Purton T, Michaelis M, Tsaousis AD. A Cell Culture Platform for the Cultivation of Cryptosporidium parvum. Curr Protoc Microbiol. 2019 Feb 8:e80. doi: 10.1002/cpmc.80.

22. Bones AJ, Jossé L, More C, Miller CN, Michaelis M, Tsaousis AD.Past and future trends of Cryptoporidium in vitro research. Exp Parasitol. 2019 Jan;196:28-37. doi: 10.1016/j.exppara.2018.12.001. Epub 2018 Dec 3.

21. Miller C. N., Panagos H., Mosedale W.R.T., Kvac M., Howard M. J. and Tsaousis A. D.*(2019) NMR metabolomics reveals effects of Cryptosporidiuminfections on host cell metabolome Gut Pathog April 3; 11:13.


20. Tsaousis A.D., Hamblin K.A., Elliot C.R., Young L., Rosell-Hidalgo A.,  Gourlay C., Moore A.L., & van der Giezen M., (2018) The Human Gut Colonizer Blastocystis Respires Using Complex II and Alternative Oxidase to Buffer Transient Oxygen Fluctuations in the Gut. Front Cell Infect Microbiol. 2018 Oct 22;8:371. doi: 10.3389/fcimb.2018.00371.

19. Miller C. N., Josse L. J., and Tsaousis A. D. (2018) Localization of Fe-S biosynthesis machinery in Cryptosporidium parvum mitosome, J Eukaryot Microbiol. 2018 Jun 22. doi: 10.1111/jeu.12663.

18. Yowang A., Tsaousis A. D., Chumphonsuk T., Thongsin N., Kullawong N., Popluechai S., Gentekaki E. (2018) High diversity and variable geographic distribution of Blastocystis subtypes isolated from adults living in Thailand. Infect Genet Evol. 2018 Aug 15;65:270-275. doi: 10.1016/j.meegid.2018.08.010.

17. Herman E. K., Yiangou L., Cantoni D. M., Miller C. N., Marciano-Carbal F., Anthonyrajah E., Dacks J. B., Tsaousis A. D., (2018) Identification and characterisation of the cryptic Golgi Apparatus in Naegleria gruberi. J Cell Sci. 2018 Apr 9;131(7).

16. Betts E. L., Gentekaki E., Thomasz A., Breakell V., Carpenter A., Tsaousis A. D. (2018) Genetic diversity of Blastocystis in non-primate animals. Parasitology, 1-7. doi:10.1017/S0031182017002347

15. Miller C. N., Josse L. J., Brown I., Blakeman, B. J. F., and Povey J. F., Nader, Price M., Cinatl J., Xue W-F., Michaelis M., and Tsaousis A. D. (2018) A new platform that enables long-term cultivation of Cryptosporidium. Int J Parasitol. 2018 Mar;48(3-4):197-201. doi: 10.1016/j.ijpara.2017.10.001.

F1000 recommendation:  


14. Rueckert S., Tsaousis A. D., (2017) Conference: Report of the 2017 Protistology-UK Spring Meeting, European Journal of Protistology, 61, 307 – 310 

13. Gentekaki, E., Curtis, B.A., Stairs, C.W., Klimeš, V., Eliáš, M., Salas-Leiva, D.E., Herman, E.K., Eme, L., Arias, M.C., Henrissat, B. Hilliou, F., Klute, M.J., Suga, H.,Malik, S.B., Pightling, W.W., Kolisko, M., Rachubinski, R.A., Schlacht, A., Soanes, D.M., Tsaousis, A. D., Archibald, J.M., Ball, S.G., Dacks, J.B., Clark, C.G., van der Giezen, M., Roger A.J. (2017) Extreme genome diversity in the hyper-prevalent parasitic eukaryote Blastocystis. PLoS Biol, 15(9):e2003769. doi: 10.1371/journal.pbio.2003769

12. Saintas E, Abrahams L, Ahmad GT, Ajakaiye AM, AlHumaidi AS, Ashmore-Harris C, Clark I, Dura UK, Fixmer CN, Ike-Morris C, Mato Prado M, Mccullough D, Mishra S, Schöler KM, Timur H, Williamson MD, Alatsatianos M, Bahsoun B, Blackburn E, Hogwood CE, Lithgow PE, Rowe M, Yiangou L, Rothweiler F, Cinatl J Jr, Zehner R, Baines AJ, Garrett MD, Gourlay CW, Griffin DK, Gullick WJ, Hargreaves E, Howard MJ, Lloyd DR, Rossman JS, Smales CM, Tsaousis A.D., von der Haar T, Wass MN, Michaelis M. Acquired resistance to oxaliplatin is not directly associated with increased resistance to DNA damage in SK-N-ASrOXALI4000, a newly established oxaliplatin-resistant sub-line of the neuroblastoma cell line SK-N-AS. PLoS One.  2017 Feb 13;12(2)



11. Richardson E., Zerr K, Tsaousis A.D., Dorrell R.G., & Dacks J.B., (2015) Evolutionary cell biology: functional insight from "endless forms most beautiful". Mol Biol Cell. 2015 Dec 15;26(25):4532-8. LINK


10. Tsaousis A.D., Nývltová E., Sutak, R., Hrdy, I., & Tachezy J. (2014) Non-mitochondial hydrogen production of Naegleria gruberi. Gen Biol Evol 6(4), 792-799. LINK

9. Tsaousis A.D., Gentekaki E., Eme L., Gaston D. & Roger A.J. (2014) Evolutionary relationships and origin of the Cytosolic Iron-Sulphur Assembly (CIA) machinery in eukaryotes. Eukaryotic Cell, 13(1): 143-53. LINK


8. Tsaousis A. D., Ollagnier-de Choudens S., Gentekaki E., Long S., Gaston D., Stechmann A., Vinella D., Py B., Fontecave M., Barras F., Lukeš J., & Roger A. J., (2012) Evolution of Fe-S cluster biogenesis in the anaerobic parasite Blastocystis, Proc Natl Acad Sci USA 109(26):10426-31. LINK



7. Tsaousis, A. D., Gaston D., Stechmann A., Walker P. B., Lithgow T. & Roger A. J. (2011) A Functional Tom70 in the Human Parasite Blastocystis sp.: Implications for the Evolution of the Mitochondrial Import Apparatus, Mol Biol Evol 28(1):781-91. LINK


6. Long S, Changmai P, Tsaousis AD, Skalický T, Verner Z, Wen YZ, Roger AJ, Lukeš J. Stage-specific requirement for Isa1 and Isa2 proteins in the mitochondrion of Trypanosoma brucei and heterologous rescue by human and Blastocystis orthologues. Mol Microbiol. 2011 Sep;81(6):1403-18. doi:

10.1111/j.1365-2958.2011.07769.x. LINK




5. Hjort, K., Goldberg, A.V., Tsaousis, A.D., Hirt, R.P., and Embley, T.M. (2010). Diversity and reductive evolution of mitochondria among microbial eukaryotes. Philos Trans R Soc Lond B Biol Sci 365, 713-727. LINK




4. Gaston D., Tsaousis A. D., & Roger A. J. (2009) Predicting proteomes of mitochondria and related organelles from genomic and expressed sequence tag data, Methods in Enzymol, 457: 21-41. LINK



3. Tsaousis, A. D., Kunji E. R. S., Goldberg, A. V., Lucocq J. M., Hirt, R. P. & Embley T. M. (2008) A novel route for ATP acquisition by the remnant mitochondria of Encephalitozoon cuniculi, Nature 453: 553-556. LINK

Faculty of 1000 Biology: evaluations for Tsaousis AD et al Nature 2008 May 22 453 (7194) :553-6 - Factor 8.0 - Exceptional


2. Goldberg, A. V., Molik, S., Tsaousis, A. D., Kuhnke, G., Delbac, F., Vivares, C., Hirt, R. P., Lill, R. & Embley T. M. (2008) Localisation and functionality of microsporidian iron-sulphur cluster assembly proteins, Nature 452: 624-628. LINK




1. Tsaousis, A. D., Martin, D. P., Ladoukakis, E. D., Posada, D. & Zouros, E. (2005) Widespread Recombination in Published Animal mtDNA Sequences, Mol Biol Evol 22: 925-933. LINK

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